種苗生産におけるBIO-PCRを用いたサツマイモ塊根内のサツマイモ基腐病菌の生死判別法の検討

抄録

Real-time PCR is highly specific and sensitive for Diaporthe destruens, which causes foot rot of sweet potato. However, PCR detects pathogens whether they are viable or not. We utilized BIO-PCR to detect viable D. destruens from sweet potato tubers. BIO-PCR increased detection sensitivity of the viable pathogen but not the nonviable. Thus, BIO-PCR could be useful to avoid false positive results, which arise from detection of nonviable pathogen.