甘藷紫紋羽病感染組織における燐酸化合物の行動と呼吸との関係

抄録

Plant tissues, when infected by certain pathogenic fungi, seems to require an abundant supply of ATP in order to synthesize organic materials necessary in their defence reaction. Such an abundant supply of ATP may be accomplished by increased respiration of infected tissue, and the production of ATP is, as generally accepted, most efficient in case the oxidative phosphorylation takes place in the respiratory process. In fact, the respiration of the Helicobasidium-infected sweet potato tissues are stimulated up to two times, when the tissues are resistant, while up to 3 to 10 times, when the tissues are susceptible. Here arises a question as to whether the extraordinarily stimulated respiration of the susceptible tissue can be effective for ATP production. A series of experiments were made to obtain an answer to this question. The results are summarized below.
Infected sweet potato tissues
resistant susceptible
Rate of respiration……increases up to 1.5 to 2 times increases up to 3 to 10 times
Rate of triphenyltetrazoliumchloride reduction do. up to 1.5 times do. up to 3 times
Q^O2_CO²/Q^N2_CO²…… 1.2 2.7
Total P…… increases by 15% increases by 20%
Ba-insoluble P…… increases by 30% increases by 30%
Ba-soluble, alcohol-insoluble.…… increases increases at first, decreases later on
(Glucose-1-phosphate, Glucose-6-phosphate Fructose-6-phophate)
Color development of section in molybdic-… ascorbic acid reagent visible after 2 or 3 days visible after 10 minutes
Ipomeamarone (an uncoupler)
in brown-discolored tissue…… +++ ++
in periphery of the above…… + ++
From these results, the writers conclude that, in the resistant tissues, the oxidative phosphorylation takes place in the stimulated respiratory process, while, in the susceptibe tissues, although the respirations are stimulated to an extraordinary extent, resultig in a rapid liberation of inorganic phosphate from phosphorylated intermediates.