1962 Volume 27 Issue 5 Pages 239-244
The present paper deals with the production of indoleacetic acid by Sclerophthora macrospora in synthetic culture media (modified Richards' solution). Indoleacetic acid was extracted from the culture filtrate with ether, and was determined by the Salkowski's color reaction, paper-chromatography, Avena straight growth test, and also by the ultraviolet absorption. As much as 0.017mg of Indoleacetic acid per 100ml of culture filtrate during 10 days culture was produced in modified Richards' solution with no addition of L-tryptophan. It increased to 0.672mg, when 0.01per cent L-tryptophan was added to the solution. When 0.1per cent L-tryptophan was added, 3.04mg of indoleacetic acid per 100ml culture filtrate was obtained.
Judging from the above results, it is considered that S. macrospora has a complete enzyme system to metabolize inorganic nitrogen to indoleacetic acid.