Abstract
When detached leaves of local lesion hosts inoculated with a high concentration of TMV were incubated at 30C for 4-5 days and then transferred to 22C, apparent necrosis did not appear on the inoculated leaves. It is suggested that necrotization does not occur in leaf tissues in which TMV has fully multiplied at 30C, even if they were then transferred to 22C, the optimal temperature for necrotization.