Tobacco Streak Virus Isolated from Tobacco Plants in Japan

Abstract

Tobacco streak virus (TSV) was isolated from Burley tobacco plants showing yellowing and veinal necrosis of leaves. Plants of 21 species from 10 genera were infected by the virus. The virus had properties of thermal inactivation point 50-55C, dilution end point 10^-3-10^-4 and longevity in vitro 12-24hr, and positively reacted with TSV antisera. Virus particles were isometric with 27-35nm in diameter. An isolate from symptomless dahlia plant was also identified as TSV. This is the first report of natural occurrence and isolation of TSV in Japan.