培地上における糸状菌の形態の経時的観察法

抄録

The following two convenient methods were devised for a successive observation of fruiting structures of intact fungi growing on artificial media. (1) Improved thin- cell-culture method: A piece of filter paper with a hole and a slit was permeated with a liquid medium and inoculated with a fungus. The preparation was held between a microscopic slide and a coverslip, and incubated at a condition favorable for fungal sporulation. Fruiting structures were observed periodically. The device was useful for light and scanning electron microscopic observation and for preparing semi-permanent mount. (2) Plastic Petri-dish method: The holes in the bottom of plastic Petri-dish were closed with coverslips, plated the agar medium, inoculated with a fungus, and incubated at a favorable condition. Microscopic observation was made through the coverslips from the bottomside of the Petri dish. This method was convenient for observation of the fruiting structures or sclerotia formed inside the medium.