1990 年 56 巻 2 号 p. 269-272
Immunofluorescent antibody technique is a useful method for detecting Phytophthora. But the autofluorescence and the nonspecific staining of soil particles interfere with the detection of the fungi in soil. This paper reports that the pretreatment of the samples with gelatin-rhodamine conjugate prevented the samples from the autofluorescence and the nonspecific staining and therefore permitted the Immunofluorescent antibody staining in soil. Stained Phytophthora was easily detected on the yellow-orange background.