Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
Stability of a Cucumber Mosaic Virus Satellite RNA Sequence Integrated into Tobacco Nuclear DNA Using Agrobacterium tumefaciens
Yoichi TAKANAMIYumiko HAYASHIChikara MASUTAShigeru KUWATAToshihiko KOMARI
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1992 Volume 58 Issue 4 Pages 522-527

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Abstract

A monomeric cDNA copy of cucumber mosaic virus (CMV) satellite RNA which is non-necrogenic to tomato was introduced into nuclear DNA of tobacco plants with the aid of the binary vector system of Agrobacterium tumefaciens. In order to check the stability of the introduced genes, kanamycin resistance and expression of biologically active satellite RNA upon infection with CMV were assayed up to the fifth generation of the homozygous progeny obtained from a primary transformant. The inserted cDNA in the progeny of the fifth generation was sequenced after cloning of the sequence amplified with the polymerase chain reaction. Neither loss nor mutations of the inserted genes were detected.

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