Serological Studies of Xanthomonas campestris pv. oryzae with Polyclonal and Monoclonal Antibodies
1993 Volume 59 Issue 2 Pages 123-127
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1993 Volume 59 Issue 2 Pages 123-127
Four polyclonal antibodies were prepared against 4 strains of Xanthomonas campestris pv. oryzae. Sixty-three strains, among which 58 came from China and 5 from International Rice Research Institute, were divided into 4 serovars (I, II, III and IV) by means of Ouchterlony double diffusion test and immunoelectrophoresis with these antibodies. Fifteen hybridoma cell lines which stably secrete monoclonal antibodies were produced through fusion of mouse myeloma cells (SP2/OAg14) and spleen cells derived from BALB/c mice immunized with whole cells of X. campestris pv. oryzae strains KS-6-6, OS-213, YZ-32 and YZ-34. The monoclonal antibodies produced by the selected 4 representative hybridoma cell lines differentiated 6 epitopes of this bacterium. By combination of these epitopes, the serovar I and II were further differentiated into 7 subserovars and 63 strains of X. campestris pv. oryzae were consequently grouped into 9 reaction types consisted of 4 serovars and 7 subserovars.
