免疫蛍光抗体法によるPseudomonas glumaeの検出

抄録

Rice plants fixed in glutalaldehyde were cut into 30μm thickness with Automatic MT-2 (Nihon-Ika). The cross sections were immersed in the fluorescent antibody labelled with FITC against Pseudomonas glumae and observed under a fluorescent microscope. Specific fluorescences derived from the bacteria were found in and on an upper and lower epidermis, in a parenchyma and in a sclerenchyma of palea and lemma of grain. They were found in an upper and lower epidermis and a vascular bundle sheath of a flag leaf blade, and around a vascular bundle and a lysigenous aerenchyma of a flag leaf sheath. These results suggest that this immunofluorescent antibody technique is useful for detecting P. glumae on rice plants.