セルトレイと植物生育調節剤を利用したイネいもち病菌レース判別試験の効率化

抄録

The method of Goto and Yamanaka (1968) was modified to improve efficiency when differentiating of Pyricularia grisea races. Three seeds of one of each of 12 Japanese differential rice varieties were sown in a 3-cm square cell of a 12-cell tray (3×4 cell) and treated immediately with 10ml of 2.5μg/ml uniconazole (a plant growth regulator) to yield dwarf seedlings. The seedlings (5-leaf stage) were then inoculated with one of 23 blast isolates (6 races), and covered with plastic cases to prevent contamination from the other isolates. The blast races identified by the method were consistent with those identified with the method of Goto and Yamanaka. With the use of dwarf seedlings and protection cases, many blast isolates can be identified at the same time.