The Detection of Candida by the Modified Imprint Culture Technique

Abstract

Imprint culture from patients with denture stomatitis is a common method used to examine the relationship between localization of yeast colonies to inflammed mucosa area. However, this technique consists of problems such as;
1. negative effect on the growth of Candida due to the temperature of the agar.
2. the need of having to box the denture base.
3. difficultly of applying this technique to partial dentures.
Therefore, a modified imprint culture technique was developed to improve these problem.
The ability to detect Candida by the modified imprint culture technique was examined in regards to temperature of the agar. Results showed that this technique did not have such an important effect on the growth of Candida.
Thus, the modified imprint culture technique was found to have many advantages such as:
1. No influence by the temperature of the agar on the growth of Candida.
2. Easy application of this technique to partial dentures.
3. Easy aseptic handling.
4. No need of a boxing process on a denture base.
5. No reinforcements of the agar.
6. A shortened length in handling time.