1972 年 11 巻 2 号 p. 187-191
Tumor ce llswere detected in stained smears with May-Grünwald-Giemsa of aspiration materials of bonemarrow in one hundred and seventy-six patientswith various malignancies other than leukemias.Specimens from primary or metastatic tumors werediagnosed histologically as adenocarcinoma in 37, anaplastic carcinoma in 13 and squamous cell carcinomain 1, respectively.
Characteristics of scattering of tumor cells onmarrow smears were observed as entirely diffuse inleukemia and other hematopoietic malignancies, diffuse with pair cell or smaller clumps of cells inanaplastic carcinoma as well as that in neuroblastoma, retinoblastoma, keratotic type of squamouscell carcinoma or mucocellular type of adenocarcinomatubulare, ca. clumping like in common typeof adenocarcinoma tubulare, that with irregular arranged, mostly firmly connected cell accumulationespecially in scirrhus type of adenocarcinoma tubulare, and that with sheet like double or triple cell layers in non-keratotic type of squamous cell carcinoma, respectively. Cells of anaplastic carcinoma, neuroblastoma or retinoblastoma were shown as homogeneous, strong or weak basophilic cytoplasm, unif ormlydistributed chromatin net-works with veryfine cr relatively fine reticular appearances, hardly visible or very smallnucleoli and increased N-Cratio such as in leukemic cells. Differential cytodiagnosis not only between anaplastic carcinoma andneuroblastoma, retinoblastoma, but hematopoieticmalignancies is infrequently difficult without close careful cytological observations, Cytomorphologicalfindings of mucocellular type of adenocarcinoma tubulare were characterized by abundant cytoplasmawith unhomogeneous basophility by various sized unstained vesicles, deformed or irregular shaped nucleuswith condensed coarse chromatin net-works and invisible nucleoli.
Cells of scirrhus type of adenocarcinoma tubularewere observed in irregular, not smoothly discriminated cell margin, increased N-C ratio, and finereticular chromatin net-works of thicker appearancesof nucleus, but less than that of anaplastic carcinoma and more than that of adenocarcinoma tubulare. Of non-keratotic type of squamous cell carcinoma, they were seen in short or long spindle or ellipsoid shaped cytoplasm with unhomogeneous weakbasophilic appearances, slightly ellipsoidal nucleuswith relatively fine, uniformly reticular chromatinnet-works and with hardly visible nucleoli. On contrary to non-keratotic type of squamous cell carcinoma, cytomorphological characteristics of its keratotic type which was obtained from metastatic bonylesion, were seen in very irregular shaped cytoplasmwith pale light blue colared and homogenous appearances like painting, irregular shaped nucleus withmarkedly condensed chromatin net-works. For theadvances in clinical practice and research in cancerproblem, cytodiagnosis must be more and more togo into details.