顕微分光測光法による子宮頸部各種病変の核DNAの解析

抄録

The following conclusions were drawn from the microspectrophotometric studies of DNA contents of various lesions of the uterine cervix.Measurements were made of the DNA contents of the specimens as follows: normal squamous epithelium (two cases);low high and equivocal dysplasias (eight cases);carcinoma in situ (three cases);invasive cancer that had carcinoma in situ lesions just above the cancer nestles (three cases).
1.In the cases of normal squamous epithelium, the predominance of diploid DNA value was characteristic of its mode, and its distribution was confined between diploid and tetraploid levels.
2.The cases of low dysplasia gave different histog rams of the layers.At the basal layer, 8 n or greater DNA values were recognized, while such irregularities were diminished as the layers went up, and finally in the fourth layer the whole histogram was confined mostly between diploid and tetraploid range.
3.In the cases of high dysplasia, the distribution of DNA was ranged further wider, spreading beyond 8n. The gradual narrowing of histograms as observed in the upper layers of the cases of mild dysplasia was absent here.However, in the case where modes were recognizable, they tended towards the lower value as the layer went up.
4.In the cases of equivocal dysplasia, histograms showed a broader range of DNA distribution, some including as much as 10n or even larger values.There was no discrepancy between the respective layers.The mode, if any, stayed on a single definite value, regar dless of layers.
5.The cases of carcinoma in situ tended to show a wider range of DNA distribution than the cases of equivocal dysplasia did.The second or the third layer gave a broader histogram than the basal layer and sometimes the fourth layer had the widest DNA distribution.And such a trend was also recognized that the values of DNA became higher as the layers went up.
6.Comparison made between the histograms of carcinoma in situ lesions that had cancer nestles developed underneath and those of carcinoma in situ lesions without invasive cancer nestles demonstrated that there was no significant difference between them.
7.From these data the following conclusions were drawn: high dysplasia and equivocal dysplasia were precancerous lesions, and carcinoma in situ, malignant lesions.
8.Comparison between the histograms of carcinoma in situ lesions and cancer nestles taken from one tissue demonstrated a clear difference in their patterns: in other words, the histograms of the invasive cancer tend to have the mode in hyperdiploid or hypertetra ploid.It appears that this may be attributed to the possible presence of stem cells in cancer of the uterine cervix as suggested by Makino.