1976 年 15 巻 2 号 p. 181-187
The degeneration process of the cancer cell was studied by using Ehrlich ascites tumor, from both morphological and biochemical points of view. As morphological methods, phase contrast microscopy was used for a raw sample, Papanicolaou staining for nuclear structure and Fast Green staining for nuclear structure as well as for quantitative analysis of histone by microspectrophotometry; and as a biochemical method, SDSpolyacrylamide gel electrophoresis was used for histone fractionation.
With the sample in a well preserved state (i.e. immediately after collection of the ascites), the nuclear structures of the cancer cells observed on the Papani colaoustaind smear were mostly the same as those on the Fast Greenstained one;however, the progression of cell degeneration, a further increase in densechromatin on the Papanicolaoustained smear, and a decrease in stainability with Fast Green were observed. It was postulated that the histone-bound phosphoric acid radicals of DNA were disclosed during cell degeneration as!free radicals which would bind to more hematoxylin in the Papanicolaou stain, whereas the drop in the isoelectric point of the nucleus during cell degeneration might reduce the staining effect of Fast Green.
A microspectrophotometric study of Fast Green reactive histone revealed that the histone decreased mostly in parallel with the morphological changes.
A follow-up study of histone fractions revealed that F1 fraction of histone disappeared in early cell degeneration.
From the result described above, it may be concluded that degeneration of the nucleus is related to the disappearance of F1 fraction of histone.