Impulse Cytophotometer (ICP) による子宮頸部各種病変に対するDNAパターンの動態について

細胞診自動化に対するアプローチ

抄録

The following conclusions were obtained from the impulse cytophotometric studies on DNA contents of specimens of various cervical lesions for the automated analysis.
The specimens were obtained by shaving the cervical lesions with a Castroriejo electro-keratotome to a depth of 0.2mm.The measurements were made for DNA contents of the cells in various cervical lesions as follows:
Normal squamous epithelium (4 cases); metaplastic epithelium (4 cases); mild dysplasia (2 cases); carcinoma in situ (4 cases);invasive carcinoma (3 cases);
1. In the cases with normal squamous epithelium, only diploid DNA value was predominant.
2. In the cases with metaplastic epithelium, the predominance of diploid DNA value with slight hyperdiploid value was characterized by its mode and its distribution was confined between diploid and hexaploid levels.
3. In the cases with mild dysplasia, the distribution of DNA contents was spreading over 8C. But, in the cases with dysplasia, the patterns of DNA contents in scraped cells were different from the mode of dysplasia for lack of dysplastic cells.
4. The cases with carcinoma in situ, it was tended to show further wider range of DNA distribution, spreading over 10C, and characteristic high level distribution from 3C to 6C. But, in the cases with carcinoma in situ, the patterns of DNA contents of scraped cells were so much influenced by other elements that stable CIS patterns could not be got from their histograms.
5. In the cases with invasive carcinoma, the range of DNA distribution was not significantly different from the range of CIS, but the hyperploid level value was lower than CIS value. In the cases with invasive carcinoma, scraped smears tended to show a wider range of DNA distribution and higher level distribution of hyperploid than the cases of cancer nestles did.
6. In the cases with invasive carcinoma, scraped smears can be utilized for automated cytology by ICP. But, in the cases with dysplasia and carcinoma in situ, scraped smears include very few cells derived from the lesions, so it needs to improve the analysis of histograms and combine a new technique to separate the hyperploid cells by flow system with cell sorter.