We describe a rare case of cystic islet cell tumor of the pancreas diagnosed by fine needle aspiration (FNA) cytology. A 63-year-old female presented with a cystic mass, measuring 8×8 cm, in the pancreatic tail. FNA yielded cystfluid containing both isolated neoplastic cells and loosely packed cell clusters. The cells had a high N/C ratio, round nuclei, stippled nuclear chromatin, prominent nucleoli, and fine granular cytoplasm. This case suggests that a stippled nuclear chromatin pattern is one of the most important findings for distinguishing islet cell tumors from acinar cell tumors and solid and cystic tumors of the pancreas.