細胞診断における固定液の再検討およびCytosprayについて

抄録

Sputum smears of lung cancer patients were kept in room after the fixation for 30minutes in 95% ethanol-ether solution, in 95% ethanol, in methanol-ether solution, in methanol only, in 90% aceton, in Bouin's solution and in Carnoy's solution respectively. They were also kept in room after the fixation during 24 hours in 10% formalin and after the spraying 2 times with Cytospray.
On the first, 2nd, 4th, 7th and 14th days after the fixation, sputum smears were stained with Papanicolaou's method. The slides, stained within 24 hours after the fixation, have good stainability of cells and nuclei, and no degeneration of cell ular structure was observed excepting 10% form alin fixed slides. The slides stained more than 24 hours after the fixation have slightly weak staina bility.
Smears fixed by several methods and stained by Papanicolaou's method, of vaginal secretion, sputum, bronchial secretion, gastric juice, urine and serous fluid are reexamined microscopically after 2 years storage. The slides, which are fixed in ethanol-ether solution or by means of Cytospray technique, reveal good preservation of color and good maintenance of cellular structure.
Merits of Cytospray technique are summarized as follows: time saving, easy handling, no cellular desquamation from the slde and no cellular contamination.