1970 年 9 巻 2 号 p. 115-119
Recently, the filtrating method has routinely been applied to the cytodiagnosis of fluids.
In this paper the Nuclepore membrane filter has been applied for cytologic study on the body fluid, urine and blood.
The Nuclepore membrane filter is composed of polycarbonate film and has uniform pore of 5 microns in diameter.
Cells collected on the smooth flat surface of the filter were well stained with the Papanicolaou method, PAS, various cytochemical methods such as acid and alkaline phosphatases, and also with the Giemsa stain without staining the filter itself. On the other hand, Millipore filter made of cellulose esters was stained with the Giemsa stain, and, thus found not suitable for the hematologic study by use of this stain.
The advantages of this Nuclepore technique were easier handling and mounting, chemical inertness optical clarity, and high yield of cancer cells with an excellent morphologic preservation.