1970 年 9 巻 2 号 p. 149-163
1. To-day, the value of the fluorescence-microscopy has been well acknowledged as a means of cytological detection for the uterine cancer and the L. von Bertalanffy method, which adopts the acridine orange as the fluorescent dye, has been in general use. Since, with this method, it takes as much as ten minutes to complete the standing so that the author intended simplify the technique. Consequently, the author invented a method by which the entire-process of staining will be finished within 15 seconds and the author named this new technique as the simplified AO (acridine orange) method.
2. Findings of various cells such as normal, malignant and so forth have been described here by this simplified AO method.
3. Classification of various cells by this simplified AO method has been carefully studied and the characteristic cellular findings in each category have been described.
4. specimen were simultaneously taken from 1062 women, and these two specimens were considered as one testing unit. In total, 1214 testing units have been investigated. Of these two, one was stained with Papanicolaou method and the other with this simplified AO method. They were independently examined and the diagnosis was made accordingly. Then, the results were carefully compared with each other. Every case was histologically studied by the punch biopsy which was taken from suspicious area with colposcopy. Of 1214 units, 71 were diagnosed as the squamose cell carcinoma of the uterine cervix, 83 were carcinoma in situ and 11 were cancer of the uterine body.
5. With this simplified AO method, 71.8% of the cases with squamose cell carcinoma were diagnosed as either class IV or V and every case without exception was diagnosed as class III or over. Likewise, 39.8% of the carcinoma in situ were diagnosed as either class IV or V and 74.7 % were reported as class III or over. No case of false negative was found with the squamose cell cancer of the cervix; but 25.3% of the carcinoma in situ were reported as false negative. These figures were almost the same with these by Papanicolaou method which were carried out simultaneously.6. This simplified AO method, not only takes very little time for both staining process and screening examination but also disclosed quite satisfactory results. Especially, marked shortening of time was noted in order to defferenciate negative specimens from positive. Also, if necessary, specimens stained with this simplified AO method can be restained with Papanicolaou method for parmanent storage. Inconclusion, the author feels this simplified AO method would be quite instrumental for detection of cancer in daily practice.