1970 年 9 巻 2 号 p. 164-169
The characteristics of complex-formation between nucleic acids and acridine orange (AO) has been studied with spectrophotometry and fluorospectrophotometry. In these experiment, the authour established improved method in fluorescence analysis by fixing the materials before staining and found the best way for detection of DNA-AO and RNA-AO complexies. Under the proper conditions, AO was demonstrated to possess distinctive conbinding characteristics forming a green fluorescence with DNA and a redfluorescence with RNA.
Ehrlich ascites carcinoma cells were fixed and stained with AO in the same manner, and the stained cells were analized fluorospectrophotometrically. The spectrum obtained, which had two peaks at 530 mμ and 620 mμ, , was successfully devided into two spectra, i.e. properly reduced spectra of DNA-AO complex and RNA-AO complex. Furthermore, the fluorescence spectra of the cells which had been treated by DNase or RNase before staining became similar to those of RNA AO complex or DNA-AO complex respectively. These results indicated that AO combined mainly with intracellular DNA and RNA at neutral pH.