日本臨床細胞学会雑誌
Online ISSN : 1882-7233
Print ISSN : 0387-1193
ISSN-L : 0387-1193
液状検体処理法の検討
小林 康夫近藤 明人矢吹 光子
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ジャーナル フリー

1970 年 9 巻 2 号 p. 170-177

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Fluid spesimens for diagnostic cytology such as pleuroperitoneal effusions, pericardial fluids, cerebrospinal fluids, urine, punctures of cysts and cystic tumors, and washings show various characteristics according to their protein or mucin contents, number of RBC, total number of cells and volume of sediments. In our study it was examined what was the most suitable method of preparing each fluid specimen of various characteristics.
1) When it is impossible to send specimens immediately after aspiration to the cytologic laboratory, the addition of formation or ethylalcohol in the ratio of 10% or 50% for each to the fluid specimen which contains no or little protein is effective for the good preservation of the cells. Especially ethylalcohol is superior in nuclear find ings of Pap-smear.
2) In the specimen containing no or little protein, it is difficult to keep cells on the slide during the process of Papanicolaou's staining. Spray fixation for May-Giemsa staining is very helpful in retaining cells on the slide.3) From the fluids containing less protein than 1.0g/dl, 80% or more cells can be gathered by centrifugation at 1, 500 r. p.m. for 5 minutes. On the fluids of more protein than 2.0g/dl, it is necessary to centrifuge at 3, 000 r.p.m. for 10 minutes for gathering 80% of all cells in the fluid.
4) Autosmear CF-12 (from Sakura Finetech nical Co. Ltd.) is the instrument for gathering the sediments on the slide glass at concurrent centrifugation. We improved its cell for the purpose of practical use on preparation of Papsmears from fluid specimens. The old cell that was of oblique axis and small funnel shaped was changed horizontal in axis and cylindrical in shape. Consequently the instrument became very useful in gathering cells especially from the fluid specimen which was little in volume (10 ml or less) and contained few cells. If the specimen was much in volume (50ml or more) and contained few cells, we used the instrument for preparing the smears from the fluid made by addition of 10ml saline solution to the sediment of centrifuged original specimen.
5) In the specimen rich in RBC, it is convenient to treat it by saponin for its hemolytic activity. Too much addition of saponin by farover the border line volume for complete hemolysis inflicts injury on cells in various degrees.
In conclusion, it has been proved that selection or combination of those methods according to the characteristics of each fluid specimen results in preparation of good smears.

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