極限環境微生物学会誌
Online ISSN : 1881-5758
Print ISSN : 1348-5474
ISSN-L : 1348-5474
原著論文
Improvement of alkaliphily of thermostable GH family 10 xylanase from Thermotoga maritima by directed evolution
W TsukimuraK WatanabeC MorokumaR YatsunamiT FukuiS Nakamura
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2010 年 9 巻 1 号 p. 15-18

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Xylanase B (XynTB) from hyperthermophilic bacterium Thermotoga maritima MSB8 is a thermostable xylanase classified into glycoside hydrolase family 10. XynTB is most active at pH 6.0, and shows lower activity at alkaline pHs. Improvement of alkaliphily of XynTB was attempted by directed evolution. One mutant enzyme that showed slightly higher activity under high temperature and alkaline pH conditions was acquired from a newly constructed random mutant library. Protein engineering study of this mutant revealed that the amino acid substitution N92D (Asn92 was substituted by Asp) could contribute to the improvement of alkaliphily.

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© 2010 Japanese Society for Extremophiles
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