Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
Rapid Screening Assay for Ripe Rot Resistance in Grape Cultivars
Mikio ShiraishiMasahiko YamadaNobuhito MitaniToshihito UenoRyoji NakauneMasaaki Nakano
Author information

Volume 75 (2006) Issue 3 Pages 264-266

Download PDF (295K) Contact us

Ripe rot caused by Colletotrichum acutatum (CA) has been the most serious preharvest disease of grape berries/clusters. Furthermore, the pathogen is highly resistant to benomyl and iminoctadine-triacetate. A rapid assay for screening ripe rot (CA) resistance of breeding materials in table grapes was developed in which the harvested berries are washed with water and surface-sterilized in ethanol. The berries were then inoculated with conidial suspensions (ca. 3.0 × 105 spores/mL per ten berries) in a polyethylene bag, and incubated at 25°C in the dark for 2 weeks. The percentage of diseased berries increased as the ripening of berries proceeded. A period of 70 to 80 days after full bloom was suitable for berry sampling and subsequent CA inoculation to evaluate varietal differences in the ripe rot resistance of grapevine.

Information related to the author
© 2006 by Japanese Society for Horticultural Science
Previous article Next article