Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
SHORT COMMUNICATION (ENGLISH)
Rapid Screening Assay for Ripe Rot Resistance in Grape Cultivars
Mikio ShiraishiMasahiko YamadaNobuhito MitaniToshihito UenoRyoji NakauneMasaaki Nakano
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Volume 75 (2006) Issue 3 Pages 264-266

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Abstract

Ripe rot caused by Colletotrichum acutatum (CA) has been the most serious preharvest disease of grape berries/clusters. Furthermore, the pathogen is highly resistant to benomyl and iminoctadine-triacetate. A rapid assay for screening ripe rot (CA) resistance of breeding materials in table grapes was developed in which the harvested berries are washed with water and surface-sterilized in ethanol. The berries were then inoculated with conidial suspensions (ca. 3.0 × 105 spores/mL per ten berries) in a polyethylene bag, and incubated at 25°C in the dark for 2 weeks. The percentage of diseased berries increased as the ripening of berries proceeded. A period of 70 to 80 days after full bloom was suitable for berry sampling and subsequent CA inoculation to evaluate varietal differences in the ripe rot resistance of grapevine.

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© 2006 by Japanese Society for Horticultural Science
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