In the present study, the standard addition method with Porapak Q column (PQ) extraction and gas chromatography-mass spectrometry analysis was adapted to quantitatively determine the character impact odorants of ‘Toyonoka’ strawberries. A significant correlation was confirmed between the contents that determine the character impact odorants in the PQ extract and the levels of standards added to the fruit samples; therefore, standard addition analysis might be a useful and reliable method to analyze already-known odorants in fruits. The concentrations of the character impact odorants, especially 2,5-dimethyl-4-hydroxy-2H-furan-3-one, hexanoic acid, γ-dodecalactone, and the esters, were detected at relatively high levels, which could explain why ‘Toyonoka’ strawberries have abundant aromas.