1972 Volume 41 Issue 3 Pages 272-280
These experiments were carried out to establish a method of anther culture of asparagus. Special efforts were made to clarify the effects of developmental stage of anthers, method of sterilization and concentration of growth regulating substances on callus and organ formation. The medium was prepared by adding 20g/l sucrose, 7.0g/l J-agar and growth regulating substances (N6-benzyladenine and NAA) to Murashige and Skoog′s medium (MS medium). The cultures were incubated at 25°C under 16 hours illumination (4, 000lux) a day with fluorescent lamps of daylight type. The experimental results are summarized as follows: