Inhibitors Concerned in the Dormancy of the Gladiolus Corm
1973 Volume 42 Issue 1 Pages 35-39
Details
1973 Volume 42 Issue 1 Pages 35-39
1) The methanol extract of gladioli corms was purified with ethyl acetate and sodium bicarbonate. The final acidic fraction was paper and column chromatographed, then three crude inhibitors were separated.
2) Inhibiting activities of inhibitors I and III were examined by the dripping of the solution on corms and by soaking cormlets in it. The inhibiting activity of inhibitor III was slightly higher than that of inhibitor I.
3) Inhibitor II was identified as abscisic acid based on its UV absorption on the paper chromatogram, the Avena coleoptile assay and its O.R.D, measurements. Abscisic acid inhibited sprouting of the bud in gladiolus cormlets at a dosage of 10-4 M.
4) Purification and identification of inhibitors I and III are being carried out. Synergistic action of inhibitors with abscisic acid is also discussed.
