1975 Volume 43 Issue 4 Pages 415-422
1. The water extract from larvae of chestnut gall wasps exhibited cytokinin activity in tobacco pith callus growth test and barley leaf chlorophyll retention test. The larvae was fractionated with ether, water and a cation exchange resin, and the activity was detected in the basic water fraction. Paper chromatographic analysis of the fraction revealed that the active substances contained in the larvae were consisted of at least two components.
2. This extract had remarkable activity for callus formation by young stems of the susceptible (var. ne-17) and resistant (var. Ginyose) chestnut trees. On Linsmaier and Skoog RM ′64 basal medium and various media containing organic substances and plant growth regulators such as 2, 4-D or kinetin as additives, chestnut stems exhibited callus formation nearly in proportion to the concentration of the larval extract added into these media. Further, the amount of callus formed by stems at the same concentrations of the added extract did not differ clearly between the susceptible and resistant varieties. The active components in the basic fraction separated by the paper chromatography promoted the callus formation by stems showing similar Rf values to those obtained in the preceding analysis.