Studies on Crosses between Distantly Related Species of Lilies

I. For the Intrastylar Pollination Technique

Abstract

1) For the purpose of overcoming cross-incompatibilities between distantly related species or hybrids of lilies, intrastylar pollinations were attempted. This technique was also used to overcome self-incompatibilities in some species or hybrids.
2) Fertilized embryos were obtained in some cases through intrastylar pollinations even when crosses were unsuccessful by stigmatic pollinations. But, in the majority of cases, these embryos were immature and very small, of less than 1mm of length and the endosperms were very unusual and were present as liquids in small quantities or sometimes almost absent.
3) In some species or hybrids, intrastylar pollinations were effective for overcoming self-incompatibilities too and a number of normal seeds were obtained.
4) In crosses between distantly related species or hybrids by intrastylar pollinations, fertilized embryos were generally obtained when a number of pollen-tubes which had passed through the basal part of the styles, remained. But fertilized embryos were not necessarily obtained even after the above passage process, so another mechanism of incompatibilities was suspected to exist aside from that which was caused by pollen-tubes stopping their growths in the style.