1977 Volume 46 Issue 2 Pages 233-244
The problem of whether species or cultivars of seeds of Allium spp., cucumber and melon, carrot, pea or garden bean could be determined by analysis of electrophoretic patterns of seed protein or seed enzymes was studied.
Electrophoresis was carried out with acrylamide gel disc.
The results obtained are summerized in Table 1.
1. Allium species: Four species of Allium were distinguished from one another by electrophoretic patterns of protein, LDH, GDH, SDH, α-GPD and CA, provided that the distinction of Welsh onion and leek or A. tuberosum was not clear in zymograms of α-GPD, and also that the distinction of Welsh onion and onion was impossible in zymograms of CA. Cultivar Hana-nira(flower-eating cultivar) in A. tuberosum showed different electrophoretic patterns in ADH, α-GPD and acid phosphatase compared with other leaf-eating cultivars.
2. Cucumber and melon: Electrophoretic patterns of protein, LDH, esterase, acid phosphatase, SDH and CA were available for separation of cucumber and melon seeds.
3. Carrot, pea and garden bean: No difference of electrophoretic patterns of protein or enzymes was observed among cultivars or groups of cultivars within these respective species.
4. Comparing the possibility of distinguishing species of several genera tested in this report II and former report I, Allium species were most easily detected with electrophoretic method, and Brassica species and Cucumis species were next in order. Comparing the protein method with the enzyme method, the latter is more useful owing to its simplicity of the handling procedures involved. Among enzymes, esterase was most widely available for the detection of many species of seeds.
