1983 Volume 52 Issue 3 Pages 223-230
The present experiment was undertaken to confirm the generally recognized assumption concerning the chimerism of Kobayashi-mikan. This cultivar is supposed to be a synthetic chimera which has arisen from the junction of satsuma mandarin (Citrus unshiu Marc.) scion and Natsudaidai (C. natsudaidai Hayata) stock. Peroxidase and esterase isozymes of foliage and fruit parts as well as immature leaves and rootlets of young seedlings were analyzed by starch gel electrophoresis. The results obtained from a zymographic comparison of Kobayashi-mikan, satsuma mandarin and Natsudaidai are summarized as follows.
1. In the case of samples taken in July, peroxidase isozyme patterns of new and old leaves as well as albedo of Kobayashi-mikan distinctly resembled those of Natsudaidai. The pattern of juice sac of Kobayashi-mikan considerably resembled that of satsuma mandarin. Esterase isozyme patterns of flavedo and albedo of Kobayashi-mikan were completely the same as those of Natsudaidai.
2. In the case of samples taken in October, peroxidase isozyme patterns of old leaves as well as flavedo of Kobayashi-mikan were nearly the same as those of Natsudaidai. The patterns of albedo and new leaves of Kobayashi-mikan resembled those of Natsudaidai. Esterase isozyme patterns of old leaves and seeds of Kobayashi -mikan were equal to those of Natsudaidai. The patterns of flavedo, alvedo and new leaves of Kobayashi-mikan were rather similar to those of Natsudaidai.
3. Peroxidase and esterase isozyme patterns of immature leaves and rootlets of young seedlings grown from seeds of Kobayashi-mikan were similar to those of young seedlings grown from seeds of Natsudaidai. From these results, the hypothesis that Kobayashi-mikan is a graft-induced periclinal chimera between satsuma mandarin and Natsudaidai has been further confirmed.