Haploid Plant Production by Anther Culture in Carrot (Daucus carota L.)

Abstract

Immature anthers of carrot (Daucus carota L.) were cultured on the Murashige and Skoog solid medium containing various combinations of phytohormones. Calli were obtained on the medium containing 0.011 mg•liter ^-1 2, 4-D and 01 mg•liter^-1 kinetin. The highest rate of callus formation (12%) was obtained on the medium containing 1 mg•liter^-1 of both 2, 4-D and kinetin. Embryoids were obtained on the medium containing 1 mg•liter^-1 2, 4-D and 00.1 mg•liter^-1 kinetin. The highest rate of embryoid formation (15%) was obtained on the medium containing 1 mg•liter^-1 2, 4-D without kinetin. Embryoids transferred to solid MS medium grew plantlets. Plants were planted to pots after acclimatization and chromosome number in root tips were observed. Among 18 plants obtained from the embryoids and calli, 16 plants were haploid (2n=9), and the other two plants were aneuploids (2n=10 and 11).