1994 Volume 63 Issue 3 Pages 537-541
This study describes the influence of the number of subcultures on the rooting ability of persimmon (Diospyros kaki L. cv. Jiro) shoots cultured in vitro, and also investigates the growth characteristics of the acclimatized plants which might be affected by rejuvenation.
1. The percentages of rooted shoots from adult plants increased with repeated subcultures. The rooting percentage was only 3.3% after three subcultures increasing to 90% after 38 subcultures. In contrast, shoots from juvenile seedlings had as high as 70% rooting after three subcultures.
2. In terms of shoot and root dry weights, the micropropagated plants grew more vigorously than seedling plants in the first growing season. Some of the micropropagated trees bore their first flower in the second growing season, suggesting that partial rejuvenation or reinvigoration, but not true rejuvenation, was responsible for the early flowering and the juvenile characteristics observed in the micropropagated plants.