Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
ISSN-L : 0013-7626
Transformation of Brussels Sprouts (Brassica oleracea var. gemmifera Zenk.) by Agrobacterium rhizogenes Harboring a Reporter, β-Glucuronidase Gene
Takashi HosokiTetsuya Kigo
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1994 Volume 63 Issue 3 Pages 589-592

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Abstract

Transfer of β-glucuronidase (GUS) gene into Brussels sprouts was made using Agrobacterium rhizogenes vector-system. Plasmid pBI 121 was transferred to A. rhizogenes IFO 13257 by the freeze-thaw method. When leaves of Brussels sprouts were inoculated with Agrobacterium, 34 hairy roows were produced with GUS activities. From seven root clones with GUS activities, 14 shoots were regenerated from calli on Murashige-Skoog (MS) medium supplemented with 1 mg•liter-1 α-naphthaleneacetic acid (NAA) plus 5 mg•liter-1 zeatin. Seven shoots obtained from four root clones again showed GUS activities, suggesting that they were transformants. In two root clones, shoots with and without GUS activity were regenerated from the same callus. Four shoots from three root clones showed no activity. Thus, half of GUS genes were stably transmitted from hairy roots to regenerated plants.

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