Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
ISSN-L : 0013-7626
Callus Formation and Plantlet Regeneration through In vitro Culture of Immature Embryo and Seedling in Chinese Chive (A Ilium tuberosum Rottler)
Hui-min XuelHajime ArakiToshinari KanazawaTakashi HaradaToshiro Yakuwa
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1997 Volume 66 Issue 2 Pages 353-358

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Abstract

This study was carried out to establish an efficient method for propagating Chinese chive (Allium tuberosum Rottler) through callus culture. Compact and white callus was in-duced from seedlings on AZ medium supplemented with 10 μM 2, 4-dichlorophenoxyace- tic acid (2, 4-D), 1 to 10 μM 2, 4-D and 0.01 to 0.1μM 6-benzyladenine (BA), and from 3-4 mm immature embryos cultures on 1 to 10 μM 2, 4-D and 0 to 10μM BA. Seedling-derived callus proliferated when subcultured on Murashige and Skoog (MS) medium containing 1, μM 2, 4-D. Higher rates of the multiple shoot formation were obtained from these proliferated calli on MS medium supplemented with 25, uM BA with or without a-naphthaleneacetic acid (NAA). Multiple shoot growth and plant regeneration rate were accelerated when shoots were transferred to a medium without plant growth regulators. Regenerated plantlets developed vigorously after being successfully accli-mated in soil.

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