2002 Volume 71 Issue 1 Pages 123-126
Shoot tips excised from the apical bud, axillary buds between the apical bud and the forth leaf (1st-4th leaf axillary bud) or axillary buds between the forth leaf and the eighth-leaf (5th-8th leaf axillary bud) were imbedded on MS medium containing 2 mg·liter-1 BA. Shoot tips excised from the apical bud developed a shoot and formed callus on the cut base, whereas those excised from axillary buds formed multiple shoots. The rate of multiple shoot formation from shoot tips of the 1st-4th leaf axillary buds was higher than that of the 5th-8th leaf axillary buds. The optimum concentration of BA in the medium for the maximum formation of multiple shoot was found to be 2 mg·liter-1 BA. The multiple shoots thus formed were proliferated efficiently by subculturing them on the medium containing 1 mg·liter-1 BA.