Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
ISSN-L : 0013-7626
Micropropagation of Shinano Walnut (Juglans regia L.)
Takuya TetsumuraKosuke TsukudaKoshiro Kawase
Author information
JOURNAL FREE ACCESS

2002 Volume 71 Issue 5 Pages 661-663

Details
Abstract

Micropropagation of three cultivars of Shinano walnut (Juglans regia L.) was performed by using DKW medium. Nodal segments (explants) produced new shoots on DKW medium supplemented with 5μM BA and 0.05μM IBA. When subcultured, shoots grew better on the above medium with agar than they did with Gelrite. There was a significant difference in the growth of shoots among the cultivars. To induce roots, IBA-treated shoots were transferred to a rooting medium, a Gelrite-solidified DKW medium with macroelements reduced to 1/4 strength, with or without vermiculite. The addition of vermiculite to the medium improved the rooting percentage and promoted the development of a good root system. All regenerated plantlets were successfully acclimatized.

Content from these authors
© Japanese Society for Horticultural Science
Previous article Next article
feedback
Top