Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
ISSN-L : 0013-7626
Phylogenetic Classification of Freesia spp. by Morphological and Physiological Characteristics and RAPD Markers
Shermarl WongchaochantMotoaki DoiHideo Imanishi
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2002 Volume 71 Issue 6 Pages 758-764

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Abstract

Morphological and physiological characteristics and RAPD makers were used to analyze the relationships among 35 accessions of Freesia, representing nine species. Cluster analysis, based on nine morphological and physiological characteristics, discriminated individual species into subgroups, that agreed with previous morphological groupings. RAPD analysis, by using 30 primers, produced 86 polymorphic bands. The phylogenetic tree, generated by UPGMA of the RAPD data, separated the accessions into eight subgroups at a genetic distance of 0.9, although two sections of Freesia could not be separated into different clusters. Accessions of F. corymbosa were clearly distinguished from F. occidentallis and F. refracta by using a RAPD marker, indicating that both analytical methods could be used for discriminating Freesia species.

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