Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
ISSN-L : 0013-7626
Effect of High Temperature on Viability and Growth of Radicles in Cucumber Seedlings
Kazuhiro DanShigeo Imada
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2002 Volume 71 Issue 6 Pages 805-811

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Abstract

To induce thermotolerance in cucumber seedlings, the effect of pre-treatment conditions were investigated by measuring the 2, 3, 5-triphenyltetrazolium chloride (TTC) staining intensity and subsequent elongation of radicles at 30°C for 2 days. When seedlings were incubated at 30, 35, 37.5, 40, 42.5 or 45°C for 2 hr, the TTC staining and subsequent elongation of radicles at 30°C for 2 days were severely inhibited after incubation at 45°C for 2 hr, indicating that radicles were damaged severely by incubation at 45°C for 2 hr. Then, we examined the pre-incubation temperature conditions to reduce the damage of radicles induced by incubation at 45°C for 2 hr. When seedlings were pre-incubated at 30, 35, 37.5, 40, 42.5 or 45°C for 2 hr prior to incubation at 45°C for 2 hr, those pre-incubated at 37.5 or 40°C incurred significantly low damage of radicles by the 2-hr 45°C treatment. The pre-incubation for more than 30 min at 37.5 or 40°C prevented the damage of radicles induced by the 2-hr 45°C treatment. These results indicate that the pre-incubation for more than 30 min at 37.5 or 40°C provides thermotolerance in cucumber radicles to subsequent high temperature stress. The degree of TTC staining and the subsequent elongation of cucumber radicles after high temperature treatments are useful indicators of high temperature damage.

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