Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
ISSN-L : 0013-7626
Plant Regeneration from Hairy Roots of Apple Rootstock, Malus prunifolia Borkh. var. ringo Asami, Strain Nagano No. 1, Transformed by Agrobacterium rhizogenes
Hiroyuki YamashitaHiroyuki DaimonYoko Akasaka-KennedyTetsuo Masuda
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2004 Volume 73 Issue 6 Pages 505-510

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Abstract

Stems of Mains prunifolia Borkh. var. ringo Asami strain Nagano No.l were infected with Agrobacterium rhizogenes MAFF-02-10265, MAFF-02-10266, MAFF-03-01724 and MAFF-03-01725 by using a needle. The frequency of hairy root formation was 2.0% with both MAFF-02-10265 and MAFF-02-10266. Transgenic plants were obtained from hairy roots that were induced by A. rhizogenes MAFF-02-10265. Adventitious shoots were formed from the hairy roots on Murashige and Skoog medium, supplemented with 5.0 mg·liter-1 6-benzyl-aminopurine (BAP), 0.1 mg·liter-1 indole-3-butyric acid (IBA) and 0.1 mg·liter-1 gibberellic acid (GA3). These shoots developed abundant roots in the culture medium. The transgenic nature of the regenerated plants was confirmed by opine and PCR analysis. A transgenic plant, acclimatized in the greenhouse, showed prolific branching, miniaturized leaves, and shortened internodes.

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