Abstract
Effects of the soluble components of cigarette smoke (TS) and the nicotine solution (NI) were investigated in vitro on the proliferation of kidney and lung cells originated from the 11th day chick embryo.
1) The inhibition of TS were greater than that of NI to the proliferation of kidney and lung cells in vitro.
2) In the present study, the results strongly suggest that TS elongates the generation time of lung cells and that the effective point of TS is different from that of NI on the cell cycle.
3) It seems that the inhibition of the proliferation on the kidney cells is caused by any other substrates in mainstream smoke except nicotine.
In conclusion, the difference between the effects of mainstream smoke and that of nicotine may be due to the difference of cell type or cell origin used in the present study. In addition, the embryonic kidney cells are effective for an inspection of the influence of the substrates except nicotine as a model of the influence of smoking to the embryo or the embryonic cells.
