Effect of Normal Syngeneic Blood Transfusion on Neuroblastoma in Mice

Abstract

The effect of the peripheral blood of normal syngeneic mice transfused to the mice of neuroblastoma (C-1300) was studied. The mice received transfusion at 48 hours after transplantation with 3×10^5 of C-1300 cells, and thereafter in every 2-3 days up to 10 doses. A single dose was consisted of 0.2 ml of peripheral blood of normal syngeneic mouse of 12-16 weeks of age. Successful rate of explant in the transfused group was 75%, being significantly lower in comparison to that of 100% in the contrast (p<0.01). The period of tumor appearance after transplantation was significantly prolonged to be 16.8±4.8 days, in comparison to 12.6±2.1 days of the contrast group (p=0.001). However, the mean survival time after appearance of the tumor was 18.8 days in the transfused group while it was 19.3 days in the contrast group. There was no significant difference. According to these results, the cause of anti-tumor effect of normal mouse blood was discussed, in relation to "natural" killer cell.