Anti-platelet and anti-atherogenic properties of eicosapentaenoic acid (EPA) are being focused. We have reported that platelet aggregation is depressed after intravenous infusion of emulsion of fish oil containing 30% EPA. We, then, manufactured an emulsion of 1, 2, 3-trieicosapentaenoylglycerol (EPA-TG). 90% of fatty acids in EPA-TG were EPA. We examined influences of two intravenous injections of the EPA-TG emulsion on platelet aggregation, serum lipids and liver function in rabbits. Platelet aggregation induced by ADP (5μM) and collagen (10μg/ml) was depressed significantly after injections. No significant changes in serum lipids and liver function were observed after injections. Our results suggest that in those patients, who can not eat anything, such as postoperative patients, EPA-TG emulsion may inhibit the initiation and progression of thrombosis.