Whole Platelet-Captured Enzyme Linked Immunosorbent Assay (WPC-ELISA) for Glycoprotein (GP) IIb/IIIa I. Establishment of the Assay and its Normal Value

Ichiro TAKEDA; Yoshihiro FUJIMURA; Yasuko MIZUMOTO; Terumi KANEHIRO; Akira YOSHIOKA; Hiromu FUKUI; Yoshiaki TOMIYAMA; Yoshiyuki KURATA; Kazuto UCHIDA

doi:10.2491/jjsth.4.69

Whole Platelet-Captured Enzyme Linked Immunosorbent Assay (WPC-ELISA) for Glycoprotein (GP) IIb/IIIa

I. Establishment of the Assay and its Normal Value

Ichiro TAKEDA, Yoshihiro FUJIMURA, Yasuko MIZUMOTO, Terumi KANEHIRO, Akira YOSHIOKA, Hiromu FUKUI, Yoshiaki TOMIYAMA, Yoshiyuki KURATA, Kazuto UCHIDA

Author information

Ichiro TAKEDA
Department of Pediatrics, Nara Medical College
Yoshihiro FUJIMURA
Department of Blood Transfusion, Nara Medical College
Yasuko MIZUMOTO
Department of Blood Transfusion, Nara Medical College
Terumi KANEHIRO
Department of Pediatrics, Nara Medical College
Akira YOSHIOKA
Department of Pediatrics, Nara Medical College
Hiromu FUKUI
Department of Pediatrics, Nara Medical College
Yoshiaki TOMIYAMA
Department of Blood Transfusion, Osaka University Hospital
Yoshiyuki KURATA
Department of Blood Transfusion, Osaka University Hospital
Kazuto UCHIDA
Nara Red Cross Blood Center

Keywords: monoclonal antibody, ELISA, GP IIb/IIIa

JOURNAL FREE ACCESS

1993 Volume 4 Issue 2 Pages 69-76

DOI https://doi.org/10.2491/jjsth.4.69

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Abstract

We describe here a new enzyme-linked Immunosorbent assay (ELISA) for the measurement of platelet glycoprotein (GP) IIb/IIIa antigen. In this assay, rabbit antihuman platelet polyclonal antibody was used for the first antibody to capture “washed whole platelets” on the polystylene immunoplate. After capturing the platelets, peroxidase-labeled anti-human platelet GPIIb/IIIa monoclonal antibody (IgG), designated as 3F11, was used for the second antibody. Using this method, platelets from 30 normal volunteers were analyzed. The mean±2SD value of GPIIb/IIIa antigen was 105.2±30.1% and the lower detection limit was 0.01%. Since ELISA does not require any specific equipments, this assay can be used in usual laboratories for the accurate determination of platelet GPIIb/IIIa antigen.

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