2018 Volume 64 Issue 6 Pages 726-732
The number of platelets in refrigerated platelet concentrate (PC) decreases due to the formation of clumps. However, when suspended in Platelet Additive Solution (PAS) as PAS-PC, the number of platelets can be maintained for long periods. Furthermore, the CD42b-positive rate reportedly decreases when refrigerated PC is re-warmed to 37°C. It is unknown whether a similar phenomenon occurs with PAS-PC. Here, we evaluated the quality of PAS-PC (65% T-PAS+/35% plasma) stored at 4°C for 21 days.
The CD62P-positive rate and annexin-V binding rate were significantly higher in refrigerated PAS-PC than PC stored at room temperature until the expiration date. The number of platelets did not significantly decrease in refrigerated PAS-PC until after 14 days and platelet aggregation was maintained for 21 days. Moreover, the mean fluorescence intensity (MFI) of CD42b and ristocetin-induced aggregation were better maintained in PAS-PC warmed after storage at 4°C than in PC stored at room temperature.
Our findings indicate that PAS-PC stored at 4°C for approximately 14 days may be clinically useful, albeit slightly activated.
