2020 Volume 66 Issue 3 Pages 538-544
Low-ionic-strength solution (LISS) is commonly used in column agglutination technology as potentiator in the detection of irregular antibody in pretransfusion testing.
In this study we evaluated the usefulness of 0.8% Ortho® Red Cell Diluent (RCD) with the ORTHO VISION® analyzer (Ortho Clinical Diagnostics Japan, Tokyo). Use of this test with an 0.8% RBC suspension (0.8% LISS-IAT) resulted in an increase in sensitivity for clinically significant antibodies and a decrease in the detection of clinically insignificant antibodies/nonspecific reactions compared to the test using LISS additive method with 4% RBC suspension (4% LISS-IAT). The 0.8% LISS-IAT detected newly produced antibodies, as early as PEG-IAT and earlier than LISS additive method. We speculate that the improvement in sensitivity with 0.8% LISS-IAT was due to a more appropriate ionic strength for antigen-antibody reaction, and efficient mixing in the reaction chamber.
We conclude that 0.8% LISS-IAT contributes to the safety of transfusion.
