Abstract
An improved method of α-D-galactosyltransferase (B transferase) assay was studied. The preceding methods were dificult to distinguish the normal and patient groups in the value of estimation. The final step in the assay istaggultinability titer count against anti-B serum after conversion to B- from H-antigen on the type O red blood cells in the incubation mixture.
The new method stands on following improved conditions:
1) The optimal concentration of reagents, i. e. substrate and metal ion in the use, were studied.
2) Use of shorter incubation period and lesser serum volume, since B-conversion is readily saturated irrespective of enzyme activity.
3) Use of selected type O red blood cells with high B-conversive capacity, so that sensitivity and reproducibility were obtained.
4) Introduction of score value, since so called titer value fails to get proportionality to the activity of the enzyme.
Adopting this method without special equipments, we are able to run a well established method with ease in the hospital laboratory use.
B transferase activity in the plasma of both normal subjects and the patients on haemodialysis were measured by this method, revealing the interesting results that the activity of patient group is significantly lower than that of normal subject group.
