1993 年 39 巻 4 号 p. 753-759
We studied antibody response against three different recombinant antigens (5-1-1/C100-3, c33c and c22-3) by RIBAII and HCV RNA detection by RT-PCR in serum samples of anti-HCV positive donors by 2nd generation HCV antibody screening test (PHA). We also compared reactivity of the two agglutination methods (PHA & PAII) with the serum samples.
In HCV antibody (PHA) positive donors, Core (c22-3) antibody was detected in 100% of PCR positive group and in 96.1% of PCR negative group whereas NS3 (c33c) antibody was detected in 98.6% of the former and in only 22.6% of the latter. The seropositivity of NS3, consequently, showed the highest correlation with PCR results among three kinds of antibodies. NS4 (5-1-1/c100-3) antibody was detected in 63.4% of PCR positive group and in 4.6% of PCR negative group. Since the majority of the elevated ALT cases were NS4 antibody positive specimens, NS4 antibody seemed to associate with some abnormality of liver function.
In comparison of PAII reactivity with PHA, PAII didn't react with a part of PHA positive (c22-3 only) PCR negative specimens, but detected all PCR positive specimens due to it's high sensitivity for NS3.