輸血と血液ガス分析

保存血の血液ガス分析ならびに血液保存における至適pHの検討

抄録

Changes in ATP content and P₅₀ of the blood during storage play an important role in oxygen transport and red blood cell viability. Three types of stored blood, namely CPD whole blood, CPD-CRC and MAP-CRC, with respect to blood gases, pH and l-lactate concentration ware examined. The number of days of storage correlated with blood acidity and l-lactate concentration, and P₅₀ correlated with pH. On the basis of these observations, the efficacy of high CO₂ permeability bags for blood preservation was tested. Blood P_CO2 remained lower and pH and P₅₀ higher on storage in these bags than on storage in conventional bags. I also examined the effects of pH change on ATP and 2, 3-DPG levels by addition of l-lactate to the stored blood. When pH of the blood was higher than 6.9, ATP level decreased significantly. When pH range was lower than 6.8, 2, 3-DPG level decreased. These results suggest that pH between 6.8 and 6.9 is optimal for the simultaneous maintenance of adequate ATP and 2, 3-DPG (and thus also P₅₀) levels.