1999 年 45 巻 4 号 p. 462-465
We prospectively evaluated the polyethylene glycol-indirect antiglobulin test (PEG-IAT) for its usefulness as a routine technique for screening unexpected antibodies against red cells. Sera of 999 consecutive parients obtained between December 1996 and March 1997 were tested by PEG-IAT in comparison with the saline, bromelin and alubumin-indirect antiglobulin (Alb-IAT) methods.
Of 999 sera, 15 contained red cell antibodies. 9 of these were reactive with both the PEG-IAT and Alb-IAT methods. 4 of the remaining 6 sera were detected only by PEG-IAT, namely 3 anti-E and 1 warm-type autoantibody. 2 cold-type anti-Leb antibodies were detected by saline and Alb-IAT, but not by PGEG-IAT.
PEG-IAT could clinically detect significant IgG-type antibodies such as anti-Rh or anti-Kidd, indicating its suitability as a routine test.